Taq DNA Polymerase, 500 Units

Taq DNA Polymerase (500 Units) is a thermostable recombinant or native enzyme isolated from Thermus aquaticus designed for routine Polymerase Chain Reaction (PCR) and DNA amplification.

Key Specifications & Features

• Enzyme Activity: 5′ → 3′ DNA polymerase activity and 5′ → 3′ exonuclease activity (lacks 3′ → 5′ proofreading exonuclease activity).
• Product Output: Generates DNA fragments with 3′-dA (adenine) overhangs, ideal for TA cloning.
• Target Length: Effectively amplifies DNA templates up to 5 kb in standard assays.
• Molecular Weight: Approximately 94 kDa single polypeptide chain.
• Package Size: 500 units total (frequently supplied at a concentration of 5 U/µL along with a compatible 10X reaction buffer and MgCl₂).
• Optimal Temperature: Active and stable at elevated temperatures (optimum polymerization around 70°C – 72°C; half-life exceeds 40 minutes at 95°C).