RPMI-1640 Medium, 500 ml

RPMI-1640 Medium (500 mL) is a widely used, sterile-filtered liquid cell culture medium originally developed at the Roswell Park Memorial Institute.

Key Features

• Primary Use: Designed for culturing human leukemic cells, lymphocytes, and a broad range of mammalian suspension and monolayer cell lines (such as HeLa, Jurkat, MCF-7, and PBMC).
• Formulation: Features a bicarbonate buffer system (typically requiring a 5%–10% CO₂ environment), amino acids, inorganic salts, high vitamin concentrations, glutathione, and often L-glutamine (depending on the specific catalog variant).
• Protein/Serum Status: Contains no proteins, lipids, or growth factors; requires supplementation with fetal bovine serum (FBS) or serum-free additives.
• Volume & Format: Supplied as a ready-to-use 500 mL sterile liquid.
• Storage: Stored refrigerated at +2 °C to +8 °C, protected from light.