Reverse Transcriptase Kit

A Reverse Transcriptase Kit is a molecular biology reagent package used to convert an RNA template into complementary DNA (cDNA) for downstream applications like PCR, qPCR, and gene expression analysis.

Key Features

• Core Enzyme: Contains a reverse transcriptase enzyme (such as MultiScribe, Quantiscript, or M-MuLV variants) that synthesizes single-stranded cDNA from total RNA or mRNA.
• Genomic DNA Removal: Many modern kits (like the QuantiTect Reverse Transcription Kit) include a rapid genomic DNA elimination or wipeout step prior to transcription.
• Primers & Buffers: Supplied with reaction buffers, dNTPs, RNase inhibitors, and a blend of primers (such as oligo-dT and random hexamers) for balanced RNA coverage.
• Fast Workflow: Standard protocols typically complete cDNA synthesis in roughly 20 to 30 minutes.